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IGFBP-6 is induced by Progesterone (P4) in T47D breast cancer cells. Asterisks indicate significance relative to the vehicle. (* means p< 0.05, ** means p<0.01, and *** means p<0.001) (A) IGFBP-6 transcriptional expression with various steroid hormone and <t>mifepristone</t> treatments (p<0.0001). V stands for ethanol, P for progesterone, E for estradiol, and R for mifepristone (RU486). (B) IGFBP-6 protein levels follow transcriptional levels (p<0.0001). (C) Representative intracellular IGFBP-6 immunoblot. (D) IGFBP-6 secretion. Units are in fg/mL/cell. IGFBP-6 concentrations are normalized by cell count (p<0.01). (E) Relative proliferation of T47D cells in response to steroid hormones (p<0.0001).
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IGFBP-6 is induced by Progesterone (P4) in T47D breast cancer cells. Asterisks indicate significance relative to the vehicle. (* means p< 0.05, ** means p<0.01, and *** means p<0.001) (A) IGFBP-6 transcriptional expression with various steroid hormone and <t>mifepristone</t> treatments (p<0.0001). V stands for ethanol, P for progesterone, E for estradiol, and R for mifepristone (RU486). (B) IGFBP-6 protein levels follow transcriptional levels (p<0.0001). (C) Representative intracellular IGFBP-6 immunoblot. (D) IGFBP-6 secretion. Units are in fg/mL/cell. IGFBP-6 concentrations are normalized by cell count (p<0.01). (E) Relative proliferation of T47D cells in response to steroid hormones (p<0.0001).
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IGFBP-6 is induced by Progesterone (P4) in T47D breast cancer cells. Asterisks indicate significance relative to the vehicle. (* means p< 0.05, ** means p<0.01, and *** means p<0.001) (A) IGFBP-6 transcriptional expression with various steroid hormone and mifepristone treatments (p<0.0001). V stands for ethanol, P for progesterone, E for estradiol, and R for mifepristone (RU486). (B) IGFBP-6 protein levels follow transcriptional levels (p<0.0001). (C) Representative intracellular IGFBP-6 immunoblot. (D) IGFBP-6 secretion. Units are in fg/mL/cell. IGFBP-6 concentrations are normalized by cell count (p<0.01). (E) Relative proliferation of T47D cells in response to steroid hormones (p<0.0001).

Journal: Frontiers in Endocrinology

Article Title: Insulin-like growth factor binding protein-6 modulates proliferative antagonism in response to progesterone in breast cancer

doi: 10.3389/fendo.2024.1450648

Figure Lengend Snippet: IGFBP-6 is induced by Progesterone (P4) in T47D breast cancer cells. Asterisks indicate significance relative to the vehicle. (* means p< 0.05, ** means p<0.01, and *** means p<0.001) (A) IGFBP-6 transcriptional expression with various steroid hormone and mifepristone treatments (p<0.0001). V stands for ethanol, P for progesterone, E for estradiol, and R for mifepristone (RU486). (B) IGFBP-6 protein levels follow transcriptional levels (p<0.0001). (C) Representative intracellular IGFBP-6 immunoblot. (D) IGFBP-6 secretion. Units are in fg/mL/cell. IGFBP-6 concentrations are normalized by cell count (p<0.01). (E) Relative proliferation of T47D cells in response to steroid hormones (p<0.0001).

Article Snippet: Cells were also treated with 25 nM Mifepristone (RU 486) (Millipore Sigma, St. Louis MO) either alone or in the presence of 50nM P4.

Techniques: Expressing, Western Blot, Cell Counting